Production of in vitro strawberry (Fragaria × ananassa) plantlets in large-scale system supplemented with silver nanoparticles

The growth of strawberry plantlets in the rooting stage on culture medium supplemented with silver nanoparticles (AgNPs) and ethylene gas accumulation plantlet bottles were investigated. In addition, different systems first used to produce large-scale Strawberry plantlets. results showed that shoots (3 cm) cultured MS 0.02 mg/L NAA, 1 g/L activated charcoal, 30 sucrose, 8 agar 0.5 AgNPs about 4 days earlier formation such as height (5.60 cm), fresh weight (242.67 mg), dry (34,67 number roots/plantlet (6.67), root length (3.40 SPAD (39.30 nmol/cm2) higher than those control after 15 culture. Besides, content bottle (0.06 ppm) treatment was lower compared (0.15 A shoot density (10 shoots) 250 mL 40 gave optimal other treatments Square plastic box system (length × width height: 19 cm 7 cm; 2.5 L volume) containing added produced 100 vigorous plantlets; meanwhile, rectangular (34 23 13 10 volume; 200 Plantlets derived from exhibited well acclimatization 60 greenhouse.